Kelapa sawit merupakan komoditas unggul pada sektor pertanian di Indonesia. Salah satu kendala pada budidaya kelapa sawit yaitu penyakit busuk pangkal batang (BPB) yang disebabkan oleh patogen Ganoderma boninense. beberapa cendawan tanah dan endofit asal tanaman kelapa sawit memiliki kemampuan dalam menghambat pertumbuhan G. boninense. Penelitan bertujuan untuk memperoleh dan mengidentifikasi cendawan tanah dan endofit asal tanaman kelapa sawit yang memiliki potensi sebagai agens pengendali G. boninense. Tahapan penelitian terdiri dari isolasi dan purifikasi cendawan tanah dan endofit asal tanaman kelapa sawit, uji patogenesitas, karakterisasi, aktivitas proteolitik, kelarutan fosfat dan uji antagonisme pada G. boninense. Hasil penelitian diperoleh 4 genus cendawan tanah non patogen yaitu Penicillium, Fusarium, Paecylomices dan Aspergillus. Cendawan endofit non patogen diperoleh 3 genus yaitu Aspergillus, Paecylomices dan Misellia Sterillia. Hasil uji kelarutan fosfat menunjukkan isolat CT3 (Aspergillus) memiliki kemampuan melarukan fosfat dengan indeks kelarutan fosfat 5,12% dan isolat CT10 (Penicillium) melarutkan fosfat dengan indeks kelarutan fosfat 2,98%. Semua isolat non patogen tidak memiliki kemampuan aktivitas proteolitik. Hasil uji antagonisme didapatkan 2 genus cendawan yang memiliki kemampuan dalam menghambat G. boninense yaitu Penicillium dan Fusarium. Hasil uji daya hambat pada genus Penicillium terdapat 2 isolat yang menghambat pertumbuhan G. boninense yaitu isolat CT2 dengan daya hambat 13% dan isolat CT4 dengan daya hambat 36,5% melalui mekanisme kompetisi dan antibiosis. Mekanisme penghambatan kompetisi ditunjukkan oleh 2 isolat yang termasuk dalam genus Fusarium yaitu isolat CT6 dengan daya hambat 35% dan isolat CT7 dengan daya hambat 35%. Berdasarkan hasil penelitian perlu dilakukan penelitian lebih lanjut mengenai isolat-isolat cendawan yang di dapatkan sebagai bahan formulasi Bio-insectisida yang aman.

Elaeis guineensis J. is the leading commodity in the agricultural sector in Indonesia. One obstacle in the cultivation of oil palm is stem rot disease (BPB) caused by the pathogen Ganoderma boninense. some soil and endophytic fungi from oil palm plants have the ability to inhibit the growth of G. boninense. This study aims to obtain and identify soil fungi and endophytes from oil palm plants that have the potential as controlling agents for G. boninense. The stages of the research consisted of isolation and purification of soil and endophytic fungi from oil palm plants, pathogenicity, characterization, proteolytic activity, phosphate solubility and antagonism test on G. boninense. The results were obtained 4 genus of non pathogenic soil fungi namely Penicillium, Fusarium, Paecylomices and Aspergillus. Non-pathogenic endophytic fungi obtained 3 genus, namely Aspergillus, Paecylomices and Misellia Sterillia. Phosphate solubility test results showed that CT3 isolat (Aspergillus) had the ability to dissolve phosphate with a phosphate solubility index of 5.12% and CT10 isolat (Penicillium) phosphate was dissolved with a phosphate solubility index of 2.98%. All non-pathogenic isolates did not have the ability for proteolytic activity. Antagonism test results obtained 2 genus of fungi that have the ability to inhibit G. boninense, that is Penicillium and Fusarium. Inhibition test results on the genus Penicillium, there were 2 isolat that inhibited the growth of G. boninense, that is CT2 isolat with 13% inhibition and CT4 isolat with 36.5% inhibition through competition mechanism and antibiotics. The mechanism of competition inhibition was demonstrated by 2 isolat included in the Fusarium genus, that is CT6 isolat with 35% inhibition and CT7 isolat with 35% inhibition. Based on the results of the study, further research is needed regarding fungal isolates obtained as ingredients for safe bio-insecticide formulations.