Salah Satu Penyakit Yang Bersifat Patogen Pada Ikan Lele Dumbo (Clarias Gariepinus) Ialah Aeromonas Hydrophila, Penyebab Penyakit Motil Aeromonas Septicemia. Penanggulangan Terhadap Penyakit Ini Sering Menggunakan Bahan-Bahan Kimia Dan Antibiotik, Sehingga Perlu Adanya Penanggulangan Yang Lebih Efektif Dan Aman Yaitu Penggunaan Bakteri Probiotik. Penelitian Ini Bertujuan Untuk Menguji Efektivitas Pemberian Bakteri Probiotik L. Achidophilus, S. Lentus Serta Kombinasi Keduanya Terhadap A. Hydrophila Secara In Vitro Dan In Vivo. Penelitian Ini Terdiri Atas Lima Perlakuan Dan Tiga Ulangan Yaitu (A) Tanpa Pemberian Probiotik Dan A. Hydrophila, (B) Pemberian Tetracyline (50 Mg/L) Dan A. Hydrophila 103 CFU/ML, (C) Pemberian L. Achidophilus 103 CFU/ML Dan A. Hydrophila 103 CFU/ML, (D) Pemberian S. Lentus 103 CFU/ML Dan A. Hydrophila 103 CFU/ML, (E) Pemberian Kombinasi L. Achidophilus 103 CFU/ML, S. Lentus 103 CFU/ML Dan A. Hydrophila 103 CFU/ML, Pemberian Bakteri Probiotik Dilakukan Setiap 2 Hari Sekali Serta Pemberian A. Hydrophila Pada Awal Pemeliharaan Dan Pengamatan Dilakukan Selama 21 Hari. Hasil Penelitian Terbaik Pada Uji In Vitro Yaitu (C) Dengan Zona Hambat 14,66±1,50 Mm. Nilai Kelangsungan Hidup Terbaik (E) 91,11±1,53%, Laju Pertumbuhan Spesifik (E) 6,00±0,05%, Konversi Pakan (E) 0,23±0,04. Pemberian Bakteri Probiotik L. Achidophilus, S. Lentus Serta Kombiasi Keduanya Melalui Media Pemeliharaan Dapat Menekan Jumlah Total Bakteri A. Hydrophila.
Kata Kunci: Ikan Lele Dumbo, L. Achidophilus, S. Lentus, A. Hydrophila, Kelangsungan Hidup

One Of The Pathogenic Diseases In African Catfish (Clarias Gariepinus) Is Aeromonas Hydrophila, The Cause Of Motile Aeromonas Septicemia. Attempting This Disease Often Uses Chemicals And Antibiotics, So There Is A Need For More Effective And Safe Countermeasures, By Use Of Probiotic Bacteria. This Study Aims To Test The Effectiveness Of The Administration Of L. Achidophilus, S. Lentus Probiotics And A Combination Of Both Against A. Hydrophila By In Vitro And In Vivo. This Study Consisted Of Five Treatments And Three Replications, Namely (A) Without The Administration Of Probiotics And A. Hydrophila, (B) Addition Of Tetracyline (50 Mg/L) And A. Hydrophila 103 CFU/ML, (C) Addition Of L. Achidophilus 103 CFU/ML And A. Hydrophila 103 CFU/ML, (D) Addition Of S. Lentus 103 CFU/ML And A. Hydrophila 103 CFU/ML, (E) Addition Of A Combination Of L. Achidophilus 103 CFU/ML, S. Lentus 103 CFU/ML And A. Hydrophila 103 CFU/ML, Administration Of Probiotic Bacteria Was Done Every 2 Days And Administration Of A. Hydrophila At The Beginning Of Maintenance And The Observation Was Carried Out For 21 Days. The Best Results In The In Vitro Test Are (C ) With Inhibition Zones Of 14.66 ± 1.50 Mm. The Best Survival Rate (E) Was 91.11 ± 1.53%, The Specific Growth Rate (E) Was 6.00 ± 0.05%, Feed Conversion (E) Was 0,23 ± 0,04. The Utilization Of L. Achidophilus, S. Lentus And Combination Of Both Through Maintenance Media Were Reduce The Total Amount Of A. Hydrophila Bacteria. Keywords: Catfish, L. Achidophilus, S. Lentus, A. Hydrophila, Survival Rate