Penyakit Vibriosis Disebabkan Oleh Serangan Bakteri Vibrio Harveyii. Penggunaan Antibiotik Dilarang Karna Memberikan Dampak Negatif Pada Larva Udang Karna Akan Meninggalkan Residu Dalam Tubuh Dan Menyebabkan Resistensi Terhadap V.Harveyii. Daun Pegagan (Centella Asiatica) Mengandung Zat Aktif Antibakteria Yang Mampu Menghambat Pertumbuhan Bakteri V.Harveyii Secara In Vitro. Penelitian Ini Bertujuan Untuk Mengetahui Daya Hambat Ekstrak C.Asiatica Dapat Menghambat Pertumbuhan Bakteri V.Harveyii Secara In Vitro.Uji Antibakteri Dilakukan Dengan Metode Difusi Kertas Cakram, Yang Terdiri Dari 5 Perlakuan, Yaitu Konsentrasi 5%, 8% Dan 10% Dan 2 Kontrol Yaitu Kontrol Positif (Tetrasiklin 0,01%) Dan Kontrol Negatif (Akuades). Hasil Senyawa Metabolit Sekunder Ekstrak C.Asiatica Dianalisis Secara Kualitatif. Pada Ekstrak C.Asiatica Mengandung Senyawa Bioaktif Antara Lain Alkaloid, Fenol Hidrokuinon, Flavonoid, Saponin, Dan Tanin. Pada Uji Antibakteri Ekstrak C.Asiatica Mampu Menghambat Pertumbuhan Bakteri Dengan Rata-Rata Diameter Zona Hambat Yang Terbentuk Dari Masing-Masing Konsentrasi Ekstrak Yaitu 5% = 14,16 Mm, Konsentrasi 8% = 16,63 Mm Dan Konsentrasi 10% = 21,44. Dari Hasil Yang Diperoleh Ekstrak C.Asiatica Menunjukkan Kemampuan Dalam Menghambat Pertumbuhan Bakteri V.Harveyii Mendekati Daya Hambat Pada Kontrol Positif (Tetrasiklin).
Kata Kunci: Daun Pegagan (C.Asiatica), Senyawa Bioaktif, V. Harveyii

Vibriosis Disease Is Caused By Vibrio Harveyii Becteria Attact. Use Of Antibiotics Is Prohibited Because It Has A Negative Impact On Shrimp Larvae Because It Will Leave The Residue In The Body And Cause Resistence To V.Harveyii. Pegagan Leaf (Centella Asiatica) Contains Antibacterial Active Substances That Can Inhibit The Growth Of V.Harveyii Bacteria In Vitro. The Purpose Of This Study Is To Determine The Effectiveness Of Leaf Extract Of C.Asiatica Can Inhibit Thegrowth Of V.Harveyii Bacteria In Vitro. The Antibacterial Test Was Performed By Disc Diffusion Method, Which Consisted Of 5 Treatments, 5% 8% And 10% And 2 Control, Is Positive Control (Tetracycline 0,01%) And Negative Control (Aquades). The Result Of Secondary Metabolite Compound Of C.Asiatica Extract Was Analyzedqualitively. In Leaf Extract Of Gotu Kola Contains Bioactive Compounds Such As Alkaloids, Phenols, Saponins And Tannins. In The Antibacterial Test Of C.Asiatica Extract Was Able To Inhibit The Growth Of Bacteria With The Average Diameter Of Inhibit Zone Formed From Each Concentration Of Extract That Is 5% = 14,16 Mm Concentration 8% = 16,63 Mm Concentration 10% = 21,44. From The Result, Obtained Leaf Extract Of C.Asiatica Showed The Abilityto Inhibit The Growth Of V.Harveyii Bacteria Close To The Drag In The Positive Control (Tetracycline). Keywords: Pegagan Leaf (C.Asiatica), Bioactive Compound, V. Harveyii