Penelitian ini bertujuan untuk mengetahui kelayakan Penuntun Praktikum dan pengaruh pemberian pupuk hayati cair yang berbasis bakteri pada pertumbuhan dan perkembangan tanaman sawi sendok (Brassica rapa sub. chinensis). Jenis penelitian yang digunakan adalah Research and Development (R&D) dengan menggunakan Borg and Gall. Penelitian ini disusun dalam rancangan acak lengkan (RAL) yang terdiri dari 4 perlakuan dan masing-masing diulang sebanyak 10 kali sehingga terdapat 40 unit percobaan. Hasil penelitian menunjukkan bahwa Perlakuan pupuk hayati cair yang diberikan pada tanaman sawi sendok berpengaruh secara signifikan terhadap parameter yang diamati pada tanaman sawi sendok. Hasil uji statistika yang dilakukan menyatakan bahwa pupuk hayati cair berpengaruh secara signifikan terhadap tinggi tanaman sawi sendok. Hasil uji kelayakan yang dilakukan pada Penuntun Praktikum Berbasis Riset diketahui memenuhi kriteria layak untuk diujicobakan ke siswa. Analisis penilaian yang diperoleh dari ahli materi sebesar 75%, ahli media sebesar 92%, guru biologi sebesar 76%. Penilaian yang diperoleh dari angket respon siswa menunjukkan bahwa 92% siswa menyatakan bahwa desain penuntun praktikum menarik.

This research is proposed to find out the feasibility of a Practical Guide and the effect of giving bacteria based liquid biofertilizer on the growth and development of spoon mustard plants (Brassica rapa sub. chinensis). The type of research used is Research and Development (R & D) using Borg and Gall. This research was arranged in a randomized design (ARD/RAL) consisting of 4 treatments and each treatment was repeat 10 times so that there were 40 units experiment. The results showed that the treatment of liquid biofertilizer given to spoon mustard plants significantly affected the parameters observed in mustard spoon plants. The results of statistical tests carried out stated that liquid biofertilizer significantly affected the height of the mustard spoon plant. The results of the due diligence carried out on the Research-Based Practicum Guide are known to meet eligible criteria for testing to students. Assessment analysis obtained from material experts was 75%, media experts were 92%, biology teachers were 76%. Assessments obtained from student response questionnaires showed that 92% of students stated that the practical guide design was interesting.