Kemampuan isolasi kandidat probiotik dari mudskipper sebagai pengendalian V. parahaemolyticus secara in-vitro. Tujuan penelitian ini adalah untuk mengetahui isolasi bakteri kandidat probiotik dari usus mudskipper terhadap tingkat pencegahan infeksi V. parahaemolyticus Secara In-vitro. Penelitian ini menggunakan 5 perlakuan 3 ulangan menggunakan isolat bakteri usus mudskipper berwarna kuning, hijau dan hitam, kontrol negatif tanpa perlakuan sedangkan kontrol positif menggunakn chloramfhenikol. Hasil penelitian menunjukkan bahwa bakteri usus mudskipper dengan warna kuning, hijau dan hitam memiliki pengaruh terhadap daya hambat pertumbuhan bakteri V. parahaemolyticus. Bakteri warna hitam dari usus mudskipper memperlihat daya hambat tertinggi pada uji zona hambat terhadap bakteri V. parahaemolyticus yaitu sebesar 33,05 mm selama 24 jam dan memiliki respon hambat pertumbuhan yang sangat kuat, dengan nilai efektivitas 75,05%. Sedangkan bakteri warna kuning dari usus mudskipper memperlihat daya hambat tertinggi pada uji zona hambat bakteri V. parahaemolyticus yaitu sebesar 43,15 mm selama 48 jam memiliki respon zona hambat pertumbuhan yang sangat kuat, dengan efektivitas 247%.
Kata Kunci: Isolasi, bakteri kandidat probiotik, usus mudskipper, V. parahaemolyticus

The ability of isolation candidate probiotic of mudskipper as a control V. parahaemolyticus In-Vitro. The purpose of this research is to know the isolation of probiotic candidate bacteria from mudskipper fish’s intestines toward the level of infection prevention of V. parahaemolyticus in an In-Vitro way. The research used 5 treatments and 3 repetitions by using yellow, green and black isolates bacteria of mudskipper fish’s intestines where negative control was not given any treatments and positive control was given chloramfhenikol. The result of the research showed that the yellow, green and black bacteria from mudskipper fish’s intestines has an effect toward growth inhibition power of V. parahaemolyticus bacteria. The black bacteria from mudskipper fish’s intestines shows the highest inhibition power on resistor zone test toward V. parahaemolyticus bacteria that is equal to 33,05 mm for 24 hours and it has a very strong growth inhibition response with the value of effectivity, 75,05%. Meanwhile, the yellow bacteria from mudskipper fish’s intestines shows the highest inhibition power on resistor zone test of V. parahaemolyticus bacteria that is equal to 43,15% mm for 48 hours and a very strong growth inhibition zone response with the effectivity, 247%. Keywords: Isolation, probiotic candidate bacteria, mudskipper fish’s intestines, V. parahaemolyticus