Tujuan penelitian untuk menghasilkan media pembelajaran Biologi menggunakan Adobe Flash CS6 materi Virus Kelas X SMA Muhammadiyah Tarakan yang layak digunakan dan untuk mendeskripsikan respon siswa terhadap pengembangan media pembelajaran Biologi menggunakan Adobe Flash CS6 pada materi Virus Kelas X SMA Muhammadiyah Tarakan. Jenis Penelitian ini merupakan penelitian Research and Development (R&D) melalui enam tahapan pengembangan yaitu Concept, Design, Material Collecting, Assembly, dan Testing. Sampel yang diambil adalah kelas X SMA Muhammadiyah Tarakan yang berjumlah 30 siswa. Hasil validasi kelayakan media menunjukan kriteria layak dengan persentase ahli media 85% kriteria layak, ahli materi 82% kriteria layak, ahli praktisi 80% kriteria layak dan hasil uji coba respon siswa menunjukan media pembelajaran Biologi yang dikembangkan sangat baik dengan persentase sebesar 93% dan dapat digunakan pada proses pembelajaran.

The research objective was to develop an Adobe Flash CS6-based Biology learning media that is suitable for use in Virus material for the tenth grade students of SMA Muhammadiyah Tarakan. This Research and Develop (R&D) study was conducted through six stages of development, namely Concept, Design, Material Collecting, Assembly, and Testing. A total of 30 tenth grade student of SMA Muhammadiyah Tarakan was taken as the study sample. The result showed that the validation score given by media experts was 85%, by material experts was 82%, by practitioner experts was 80%, nd students’ responses through the media trial showed a very good category (93%). It was concluded that the biology learning media developed based on Adobe Flash CS6 software for virus material was suitable for use for the tenth grade students of SMA Muhammadiyah Tarakan.