Penelitian ini bertujuan untuk mengetahui profil kemampuan Technological Pedagogical and Content Knowledge (TPACK) pada guru Biologi di Kota Tarakan dalam pelaksanaan pembelajaran ditinjau dari lama mengajar. Metode penelitian berupa survey dengan pendekatan kuantitatif. Subjek penelitian yaitu 9 guru Biologi SMA/MA di Kota Tarakan dengan penentuan sampel menggunakan teknik purposive sampling. Data diperoleh berdasarkan hasil kuesioner tertutup dan observasi. Hasil penelitian diperoleh nilai rata-rata kemampuan guru biologi dengan lama mengajar < 6 tahun pada aspek Technological Knowledge (TK) sebesar 69,47% tergolong baik, aspek Pedagogical Knowledge (PK) sebesar 61,1% tergolong baik, aspek Content Knowledge (CK) sebesar 47,22% tergolong cukup. Guru biologi dengan lama mengajar 6 -10 tahun nilai rata-rata kemampuan pada aspek Technological Knowledge (TK) sebesar 100% tergolong sangat baik, aspek Pedagogical Knowledge (PK) sebesar 100% tergolong sangat baik, aspek Content Knowledge (CK) sebesar 100% tergolong sangat baik. Guru biologi dengan lama mengajar > 10 tahun nilai rata-rata kemampuan pada aspek TechnologicalKnowledge (TK) sebesar 66,7% tergolong baik, aspek Pedagogical Knowledge(PK) sebesar 72,22% tergolong baik, aspek Content Knowledge (CK) sebesar 66,65% tergolong baik, Berdasarkan hasil penelitian, diketahui bahwa lama mengajar berpengaruh pada kemampuan guru pada aspek Pedagogical Knowledge (PK) dan Content Knowledge (CK), sedangkan pada aspek Technological Knowledge (TK) lama mengajar tidak berpengaruh.

Kata Kunci: TPACK, Lama Mengajar

The purpose of the research was to determine the profile of Technological Pedagogical and Content Knowledge (TPACK) abilities of Biology teachers in Tarakan City in the implementation of learning in terms of teaching experience. The research method was a survey with a quantitative approach. The research subjects were 9 Biology teachers of SMA/MA in Tarakan City. The sampling technique used was purposive sampling. The data were obtained from the results of a closed questionnaire and observation. The results showed that the average of biology teachers with less than six years of teaching experience had technological knowledge (TK) scores that were 69.47 % with good category, pedagogical knowledge (PK) scores that were 61.1 % with good category, and content knowledge (CK) scores that were 47.22 % with sufficient category. Biology teachers with the teaching experience of 6 to 10 years had the average value of the Technological Knowledge (TK) scores of 100% with very good category, the Pedagogical Knowledge (PK) scores of 100% with very good category, the Content Knowledge (CK) scores of 100% with very good category. Biology teachers with the teaching experience > 10 years, the average value of ability in the Technological Knowledge (TK) scores of 66.7 % with good category, the Pedagogical Knowledge (PK) scores of 72.22 % with good category, the Content Knowledge (CK) scores of 66.65 % with good category. The results of the research shows that the teaching experience has affected the teacher's abilities in the aspects of Pedagogical Knowledge (PK) and Content Knowledge (CK), while in the aspect of Technological Knowledge (TK) it doesn't have an effect. Keywords: TPACK, Teaching Experience