Tanaman Jagung (Zea Mays L.) Merupakan Tanaman Pangan Yang Sangat Penting Karena Hingga Saat Ini, Jagung Merupakan Makanan Pengganti Beras Sebagian Penduduk Indonesia. Hal Ini Menyebabkan Permintaan Jagung Semakin Meningkat Seiring Dengan Meningkatnya Teknologi Pengolahan Tanaman Jagung Sebagai Bahan Baku Makanan, Minuman, Pakan Ternak, Serta Bahan Industri Lainnya. Peningkatan Produksi Tanaman Jagung Dapat Dipengaruhi Oleh Faktor Eksternal Seperti Ketersediaan Nutrisi, Hormon Pemacu Pertumbuhan Serta Keberadaan Patogen Di Daerah Rhizosfer.
Aplikasi Cendawan Rhizosfer Diketahui Mampu Meningkatkan Pertumbuhan Tanaman Jagung Baik Secara Langsung Maupun Tidak Langsung. Laboratorium Perlindungan Tanaman Fakultas Pertanian Universitas Borneo Tarakan Memiliki Sembilan Koleksi Isolat Cendawan Rhizosfer Yang Diketahui Mampu Melarutkan Fosfat, Memiliki Aktifitas Protiolitik Dan Menghambat Fusarium Oxysporum.
Penelitian Ini Bertujuan Untuk Mengetahui Isolat Cendawan Yang Dapat Digunakan Sebagai Pemacu Pertumbuhan Jagung (Zea Mays L.) Dan Untuk Mengetahui Kemampuan Isolat Cendawan Dalam Menghidrolisis Kitin Dan Menghasikan Metabolit Sekunder Sebagai Nematisida M. Incognita. Rancangan Penelitian Yang Digunakan Adalah Rancangan Acak Lengkap (RAL) Dan Rancangan Acak Kelompok (RAK). RAL Digunakan Pada Pengujian Aktifitas Fisiologi (Uji Kemampuan Hidrolisis Kitin Serta Uji Kemampuan Metabolit Sekunder Dan Cendawan Sebagai Nematisida) Secara In Vitro Yang Dilaksanakan Pada Kondisi Lingkungan Yang Homogen Dan Terkontrol Sedangkan RAK Ditarapkan Pada Percobaan Yang Dilakukan Pada Lingkungan Tidak Homogen. Parameter Penelitian Yang Dirancang Menggunakan RAK Diantaranya Tinggi Tanaman, Panjang Daun, Jumlah Daun, Diameter Batang, Pengukuran PH Tanah, Panjang Akar, Bobot Basah Dan Bobot Kering. Dalam Percobaan RAK Setiap Unit Percobaan Ditempatkan Secara Acak Pada Setiap Kelompok Dengan Tiga Ulangan. Adapun Perlakuan Penelitian Adalah: K0 (Kontrol Aqudes Steril), K1 (Kontrol PDB (Potato Dextrose Broth)), P1 (Isolat C1(10)), P2 (Isolat C1(11)), P3 (Isolat C1(12)), P4 (Isolat C1(13)), P5 (Isolat C1(20)), P6 (Isolat C(24)), P7 (Isolat C(28)), P8 (Isolat C(29)), P9 (Isolat C(31)).
Hasil Penelitian Menunjukkan Bahwa Terdapat Lima Isolat Cendawan Yang Memiliki Kemampuan Meningkatkan Pertumbuhan Tanaman Dan Aktivitas Fisiologis Lainnya. Berdasarkan Hasil Tersebut Dapat Disimpulkan Bahwa Isolat Cendawan C31, C24, C1(10), C1(13), C1(20) Memiliki Kemampuan Memacu Pertumbuhan Tanaman Jagung (Zea Mays L.) Kelima Cendawan Tersebut Berpengaruh Positif Meningkatkan Tinggi Tanaman, Jumlah Daun, Panjang Daun, Diameter Batang, Jumlah Klorofil, Bobot Basah Dan Bobot Kering Serta Mampu Meningkatkan PH Tanah. Isolat Cendawan C1(10), C1(11), C1(12), C1(13), C1(20), C(29) Memiliki Kemampuan Menghidrolisi Kitin Dan Menghasilkan Senyawa Yang Dapat Mencegah Pertumbuhan M. Incognita, Kemampuan Cendawan Menghasilkan Metabolit Sekunder Sebagai Nematisida M. Incognita Dimiliki Oleh Semua Isolat Cendawan Yang Diuji Pada Penelitian Ini. Perlu Dilakukan Penelitian Lebih Lanjut Untuk Mendapatkan Formulasi Sebagai Biofestilizer.

Plant Corn (Zea Mays L.) Is A Very Important Food Crops Because Until Now, Corn Is A Food Substitute For Rice Of Indonesia. This Causes The Demand For Corn Increases With Increasing Crop Processing Technology Of Maize As Raw Food, Beverages, Animal Feed, And Other Industrial Materials. The Increase In Production Of Corn Plants Can Be Affected By External Factors Such As The Availability Of Nutrition, Growth Hormone Boosters As Well As The Presence Of Pathogens In The Rhizosfer Area. Application Of Fungi Rhizosfer Known To Increase Corn Plant Growth Either Directly Or Indirectly. Plant Protection Laboratory Of The Faculty Of Agriculture University Borneo Tarakan Had Nine Collection Of Isolates Of Fungi Rhizosfer Known Is Able To Dissolve Phosphate, Proteolityc Activity, And Inhibits Fusarium Oxysporum. This Research Aims To Know The Fungi Isolates Can Be Used As Boosters Of Growth Of Maize (Zea Mays L.) And To Know The Ability Of Isolates Of Fungi In Hydrolysis Chitin And Produce Secondary Metabolites As Nematisida M. Incognita. Research Design Used Was Complete Random Design (RAL) And The Random Design Group (RAK). RAL Used In Testing The Physiology Activities (Test The Ability Of The Hydrolysis Of Chitin And Test The Ability Of Secondary Metabolites And Fungi As Nematisida) For In Vitro Fertilization Are Carried Out On Homogeneous Environmental Conditions And Controlled While The RAK Applied To Experiments Conducted In The Environment Is Not Homogeneous. The Parameters Of The Research Which Is Designed To Use RAK Including Plant Height, Leaf Length, Number Of Leaves, Stem Diameter, Soil PH, Measuring The Length Of The Roots, The Weight Of Wet And Dry Weights. In The Experiment The Experiment Each Unit RAK Are Placed Randomly On Each Group With Three Replicates. As For The Treatment Of The Research Are: K0 (Control Aqudes Sterile), K1 (Control Of PDB (Potato Dextrose Broth)), P1 (Isolates C1(10)), P2 (Isolates C1(11)), P3 (Isolates C1(12)), P4 (Isolates C1(13)), P5 (Isolates C1(20)), P6 (Isolates C(24)), P7 (Isolates C(28)), P8 (Isolates C(29)) , P9 (Isolates C(31)). The Results Showed That There Were Five Isolates Of Fungi Are Have The Ability Increase Plant Growth And Physiological Activity Of Others. Based On These Results It Can Be Concluded That Isolates Fungi C(31), C(24), C1(10), C1(13), C1(20) Has The Ability To Spur Plant Growth Of Corn (Zea Mays L.) The Positive Effect Of Fungi Fifth Increase Plant Height, Number Of Leaves, The Length Of The Leaves, Stem Diameter, The Amount Of Chlorophyll, Wet Weights And Dry Weights Was Able To Increase The PH Of The Soil. Isolates Of Fungi C1(10), C1(11), C1(12), C1(13), C1(20), C(29) Hydrolyze Chitin And Had The Ability To Produce Compounds That Can Prevent The Growth Of M. Incognita, The Ability Of Fungi Produce Secondary Metabolites As Nematiside M. Incognita Owned By All Isolates Tested Fungi In This Research. Further Research Needs To Be Done To Get The Formulation As Biofertilizer.