Penelitian ini bertujuan untuk menganalisis kemampuan TPACK (Technological Pedagogical and Content Knowledge) pada guru Biologi SMA/MA selama pembelajaran daring di Kota Tarakan. Metode penelitian berupa survey dengan pendekatan kuantitatif. Subjek penelitian yaitu 8 guru Biologi SMA/MA di Kota Tarakan dengan penentuan sampel menggunakan teknik cluster sampling. Data diperoleh berdasarkan hasil kuesioner tertutup dan wawancara. Hasil penelitian didapatkan nilai rata-rata persentase kemampuan Technological Knowledge (TK) guru Biologi sebesar 57,8%, Pedagogical Knowledge (PK) guru Biologi sebesar 65,6%, Content Knowledge (CK) guru Biologi sebesar 67,1%, Technological Content Knowledge (TCK) guru Biologi sebesar 57,8%, Pedagogical Content Knowledge (PCK) guru Biologi sebesar 57,8%, Technological Pedagogical Knowledge (TPK) guru Biologi sebesar 59,3%, dan Technological Pedagogical and Content Knowledge (TPACK) guru Biologi sebesar 59,4%. Berdasarkan hasil penelitian dari analisis data, dapat diketahui bahwa kemampuan Technological Pedagogical and Content Knowledge (TPACK) guru Biologi SMA/MA selama pembelajaran daring di Kota Tarakan tergolong cukup dengan nilai rata-rata persentase sebesar 59,4%.

This research aimed to analyze SMA/MA Biology teacher’s TPACK (Technological Pedagogical and Content Knowledge) ability during online learning in Tarakan. The research used survey method and quantitative approach. The research subjects were 8 Biology teachers of SMA/MA in Tarakan and the samples were determined using cluster sampling technique. The data were obtained through closed questionnaire and interview. The research results showed the average percentage value of the Biology teacher’s ability in Technological Knowledge (TK) of 57,8%, Pedagogical Knowledge (PK) of 65,6%, Content Knowledge (CK) of 67,1%, Technological Content Knowledge (TCK) of 57,8%, Pedagogical Content Knowledge (PCK) of 57,8%, Technological Pedagogical Knowledge (TPK) of 59,3%, and Technological Pedagogical and Content Knowledge (TPACK) of 59,4%. Based on the research results from data analysis, it was found that the SMA/MA Biology teacher’s Technological Pedagogical and Content Knowledge (TPACK) ability during online learning in tarakan was classified with average percentage value of 59,4%. Keywords: Analysis, TPACK, Online learning