Hakikat sains (Nature of Science) merupakan ilmu pengetahuan yang merujuk pada epistimologi (metode) sains, proses, nilai dan keyakinan yang melekat pada pengembangan suatu ilmu pengetahuan sains. Penelitian ini bertujuan untuk mengetahui pemahaman hakikat sains mahasiswa baru angkatan 2021 Program Studi Pendidikan Biologi di Universitas Borneo Tarakan. Jenis penelitian ini adalah deskriptif dengan metode kualitatif. Teknik pengumpulan data dilakukan menggunakan teknik sampling kuota, dengan jumlah sampel sebanyak 48 mahasiswa dari keseluruhan populasi 59 mahasiswa. Instrumen penelitian ini adalah VNOS form B yaitu soal tes I dan soal tes II terkait hakikat sains yang berisi enam item soal mewakili setiap aspek pada hakikat sains diantaranya Tentativ, Empiris Base, Theory And Law, Social And Culture, Creativty, serta Observation And Inferense. Kategori presentase dalam penelitian ini termasuk dalam kategori sangat rendah dengan nilai

Nature of science is the knowledge that refers to the science epistemology (method), process, value, and belief that is attached to the development of scientific knowledge and about how to deal with and solve scientific problems wisely. This research has a purpose to find out about the nature of scientific knowledge of new student batch 2021 from the Biology Education Program Study at Borneo Tarakan University. This type of research is descriptive with the qualitative method. The data collection technique is done by quota sampling technique, with a number of samples of as many as 48 students from the whole population, about 59 students. This research instrument is VNOS form B, which consists of test questions I and test questions II related to the nature of science that consists of six question items that represent every aspect of the nature of science, such as Tentative, Empiris Base, Theory And Law, Social And Culture, Creativity, and also Observation And Inference. The percentage category of this research belongs to the very low category with the value around