Penggunaan nematoda entomopatogen sebagai agens pengendali hayati masih terbatas penggunaanya. hal ini disebabkan alternatif media pembawa untuk aplikasi umumnya hanya menggunakan air. penelitian ini bertujuan (1) mengetahui jenis media pembawa nep yang dapat digunakan untuk aplikasi; dan (2) membandingkan efektifitas media pembawa apakah yang efektif digunakan untuk aplikasi nep. metode yang digunakan pada penelitian ini yakni metode eksperimen dengan perlakuan: (1) media air; (2) media spons cair; (3) media t. mollitor; (4) media tepung kedelai ditambah dog food dan 1 larva terinfeksi nep; (5) media tepung jagung ditambah dog food dan larva terinfeksi nep; dan (6) media tepung jagung ditambah limbah kulit udang dan larva terinfeksi nep. masing unit percobaan diulang sebanyak 7 kali. hasil penelitian menunjukkan tingkat mortalitas tertinggi (80%) pada media pembawa spons cair, namun tidak terdapat perbedaan tingkat mortalitas antar media pembawa. selain itu, media spons cair memiliki jumlah infective juvenile (ij) tertinggi (56,8 ij), dan berbeda nyata hanya dengan media tepung jagung ditambah limbah kulit udang dan larva terinfeksi nep. penggunaan media air, spons cair, dan larva terinfeksi dapat menjadi alternative untuk aplikasi nep, sedangkan penambahan tepung limbah udang tidak direkomendasikan karena diduga mempegaruhi aktivitas nep terhadap inang, sehingga perlu dikaji kembali. kata kunci: media pembawa, nematoda entomopatogen, spons cair

The use of entomopathogenic nematode as a biological control agent is still limited. this is because an alternative carrier medium for application generally only uses water. this research aimed to ( 1 ) figure out the types of nep carrier media possibly used for application; and ( 2 ) compare the affectiveness of carrier media used for nep application. the research used an experimental method with the following treatments: ( 1 ) water media; ( 2 ) liquid sponge media; ( 3 ) t. mollitor media; ( 4 ) soybean flour media added with dog food and nep-infected larvae; ( 5 ) corn flour media added with dog food and nep-infected larvae; and ( 6 ) corn flour media added with shrimp shell waste and nep-infected larvae. each experimental unit was repeated 7 times. the results show the highest mortality rate ( 80% ) in the liquid sponge carrier media, yet there was no difference in the mortality rate between the carrier media. in addition, the liquid sponge media had the highest number of infective juveniles (ij), and significance different only with corn flour media added with shrimp shell waste and nep-infected larvae. the use of water media, liquid sponge, and infected larvae could be an alternative for nep application, while the addition of shrimp waste flour was not recommended since possibly affected the nep activity to the host, so a review was greatly required. keywords: carrier media, entomopathogenic nematode, liquid sponge