Ikan lele merupakan salah satu komoditas yang sangat diminati masyarakat dan ikan lele juga memiliki permintaan pasar yang cukup tinggi. upaya dalam peningkatan produksi ikan lele melalui subsektor perikanan budidaya dapat dicapai dengan adanya penyediaan benih yang cukup tahan dan berkualitas. penelitian ini bertujuan untuk mengetahui pengaruh dari uji tantang pada benih ikan lele tanpa pemberian pakan pasca pemijahan dengan induk yang diberi tepung kunyit (curcuma domestika) dan hormon tiroksin. uji tantang yang dilakukan pada penelitian ini menggunakan benih ikan lele berumur 3 hari. penelitian terbagi menjadi dua tahapan, tahapan pertama yaitu pemberian dosis (4,8 gram tepung kuyit, 0,1 mg horomon tiroksin). tahapan kedua yaitu pengujian dosis. uji tantang pada benih ikan lele dilakukan selama 12 hari. parameter yang diukur selama penelitian yaitu kelangsungan hidup, dan pertumbuhan benih ikan lele, serta parameter kualitas air (ph, suhu, do, dan amoniak). hasil penelitian menunjukkan kelangsungan hidup benih ikan lele pada pemijahan induk yang diberi tepung kunyit (4,8 gram) sebesar 42% dan hormon tiroksin (0,1 mg) sebesar 45%, sedangkan perlakuan pada kontrol sebesar 45%. hasil pertumbuhan pada benih ikan lele menunjukkan hasil yang tidak berbeda jauh antar perlakuan. sedangkan, hasil pengukuran kualitas air selama penelitian didapatkan hasil pengukuran yang masih tergolong baik bagi benih ikan lele (clarias gariepinus). kata kunci: hormon tiroksin, tepung kunyit, benih ikan lele

Catfish is one commodity that is in great demand by the public and catfish also has a fairly high market demand. efforts to increase catfish production through the aquaculture sub-sector can be achieved by providing sufficient quality and resistant seeds. this study aimed to determine the effect of the challenge test on catfish fry without post-spawning feed with parent given turmeric flour (curcuma domestica) and thyroxine hormone. the challenge test was carried out in this study using 3 days old catfish fry. the study was divided into two stages, the first stage was dosing (4.8 grams of turmeric flour, 0.1 mg of thyroxine hormone). the second stage is testing the dose. challenge test on catfish fry was carried out for 12 days. parameters measured during the study were survival and growth of catfish fry, as well as water quality parameters (ph, temperature, do, and ammonia). the results showed that the survival of catfish fry in broodstock given turmeric flour (4.8 grams) was 42% and thyroxine hormone (0.1 mg) was 45%, while the control treatment was 45%. growth results on catfish seeds showed results that did not differ much between treatments. meanwhile, the results of water quality measurements during the study showed that the measurement results were still relatively good for catfish fry (clarias gariepinus). keywords: thyroxine hormone, turmeric flour, catfish seeds