Tujuan penelitian untuk menghasilkan media pembelajaran biologi menggunakan adobe flash cs6 materi virus kelas x sma muhammadiyah tarakan yang layak digunakan dan untuk mendeskripsikan respon siswa terhadap pengembangan media pembelajaran biologi menggunakan adobe flash cs6 pada materi virus kelas x sma muhammadiyah tarakan. jenis penelitian ini merupakan penelitian research and development (r&d) melalui enam tahapan pengembangan yaitu concept, design, material collecting, assembly, dan testing. sampel yang diambil adalah kelas x sma muhammadiyah tarakan yang berjumlah 30 siswa. hasil validasi kelayakan media menunjukan kriteria layak dengan persentase ahli media 85% kriteria layak, ahli materi 82% kriteria layak, ahli praktisi 80% kriteria layak dan hasil uji coba respon siswa menunjukan media pembelajaran biologi yang dikembangkan sangat baik dengan persentase sebesar 93% dan dapat digunakan pada proses pembelajaran.

The research objective was to develop an adobe flash cs6-based biology learning media that is suitable for use in virus material for the tenth grade students of sma muhammadiyah tarakan. this research and develop (r&d) study was conducted through six stages of development, namely concept, design, material collecting, assembly, and testing. a total of 30 tenth grade student of sma muhammadiyah tarakan was taken as the study sample. the result showed that the validation score given by media experts was 85%, by material experts was 82%, by practitioner experts was 80%, nd students’ responses through the media trial showed a very good category (93%). it was concluded that the biology learning media developed based on adobe flash cs6 software for virus material was suitable for use for the tenth grade students of sma muhammadiyah tarakan.