UPT. Perpustakaan Universitas Borneo Tarakan | ELECTRONIC THESES AND DISSERTATION
Image of Seleksi Rhizobakteri Indigenous Sebagai Agens Pemacu Pertumbuhan Tanaman Jagung (Zea mays L.)

Seleksi Rhizobakteri Indigenous Sebagai Agens Pemacu Pertumbuhan Tanaman Jagung (Zea mays L.)

Pengarang : Dian Arif Al Maliki

Perpustakaan UBT : Universitas Borneo Tarakan,2019
    SKRIPSI

Abstrak Indonesia

Tanaman jagung (zea mays l.) merupakan komoditas pangan paling penting setelah padi yang banyak diusahakan di indonesia, seiring meningkatnya jumlah penduduk permintaan jagung juga semakin meningkat. peningkatan produksi tanaman jagung dapat dipengaruhi oleh beberapa hal seperti, ketersediaan unsur hara, adanya hormon pemacu pertumbuhan, serta keberadaan bakteri di daerah rhizosfer. aplikasi rhizobakteri diketahui mampu meningkatkan pertumbuhan tanaman jagung baik secara langsung maupun tidak langsung. laboratorium perlindungan tanaman fakultas pertanian universitas borneo tarakan memiliki koleksi 35 isolat rhizobakteri yang diisolasi dari perakaran tanaman sawi di daerah tarakan. rhizobakteri ini ada yang mampu melarutkan fosfat, memproduksi enzim protease serta bersifat antagonis terhadap rhizoctonia solani. tujuan penelitian ini adalah untuk mendapatkan rhizobakteri yang mampu digunakan sebagai pemacu pertumbuhan tanaman dan mengetahui karakteristik isolat yang mampu menambat nitrogen, mampu berpendar dan resisten terhadap antibiotik. rancangan penelitian yang digunakan adalah rancangan acak kelompok (rak). parameter penelitian yang diamati mengunakan rancangan acak kelompok yaitu, tinggi tanaman, panjang daun, jumlah daun, diameter batang, panjang akar, bobot basah, bobot kering, dan ph tanah. tiap unit percobaan ditempatkan secara acak pada setiap kelompok ada tiga ulangan dan 37 perlakuan. perlakuan dalam penelitian ini adalah k1 (kontrol air), k2 (kontrol nb), b1(7), b1(9), b1(13), b1(17), b1(19), b1(20), b2(6), b3(2), b3(9), b3(10), b3(11), b3(12), b3(16), b3(19), b4(3), b4(5), b4(6), b4(8), b4(11), b4(12), b4(13), b4(15), b4(17), b5(2), b5(4), b5(6), b5(8), b5(9), b5(10), b5(12), b5(13), b5(14), b5(15), b5(19), b3(7). hasil penelitian menunjukkan bahwa terdapat lima isolat rhizobakteri yang memiliki kemampuan memacu pertumbuhan tanaman, yaitu isolat rhizobakteri b5(4), b5(8), b4(12), b4(13), dan b3(16). kelima isolat rhizobakteri tersebut mempunyai pengaruh dalam meningkatkan tinggi tanaman, jumlah daun, panjang daun, diameter batang, jumlah klorofil, bobot basah, bobot kering, serta mampu meningkatkan ph tanah. isolat rhizobakteri b1(9), b1(13), b1(17), b1(20), b3(10), b3(12), b3(16), b4(12), b4(13), b4(5), b4(6), b4(8), b5(10), b5(14), b5(4) dan b5(8) mampu melakukan aktivitas berpendar. isolat b1(7), b1(19), b3(10), b3(12), b3(16), b3(19), b3(9), b4(12), b4(15), b4(5), b5(13), b5(15), b5(19), b5(8), b5(9) mampu menambat nitrogen. isolat b1(17), b1(7), b2(6), b3(10), b3(12), b3(19), b4(12), b4(15), b4(3), b5(10), b5(13), dan b5(4) mampu resisten terhadap antibiotik chloramphenicol pada dosis 75 Μg ml-, dan isolat b1(13), b1(20), b2(6), b3(12), b3(19), b3(2), b3(9), b4(11), b4(13), b4(15), b4(3), b5(14), b5(15), dan b5(2). resisten terhadap antibiotik amoxilin dan rifampicin pada dosis 50 Μg ml-1.

Abstrak Indonesia

Plant corn (zea mays l) is the most important food commudity after rice is widely sought in indonesia, along with the increasing number of population resulting in increasing demand for corn, therefore corn production must be increased in order to meet food needs in indonesia. the increase in corn production can be influenced by several things, such as the availability of nutrients, the growth hormone, and the presence of bacteria in the rhizosphere. rhizobacteria application is known to be able to increase the growth of corn plants both directly and indirectly. the plant protection laboratory of the faculty of agriculture, university of borneo tarakan has a collection of 35 rhizobacterial isolates isolated from the roots of mustard plants in the tarakan area. rhizobacteria is known to be able to dissolve phosphate, produce the enzyme protease and antagonist against rhizoctonia solani. the purpose of this study was to obtain rhizobacteria that can be used as a trigger for plant growth and to determine the characteristics of isolates capable of tethering nitrogen, being able to fluoresce and the risk of antibiotics. the study design used was randomized block design (rbd), the research parameters were observed using a randomized block design, namely, plant height, leaf length, number of leaves, stem diameter, root length, wet weight, dry weight, and soil ph. the procedure of the study using randomized design the groups of each research unit were placed randomly in each group with three repetitions and 37 treatments. the treatment in this study was k1 (water control), k2 (nb control, b1(7), b1(9), b1(13), b1(17), b1(19), b1(20), b2(6), b3(2), b3(9), b3(10), b3(11), b3(12), b3(16), b3(19), b4(3), b4(5), b4(6), b4(8), b4(11), b4(12), b4(13), b4(15), b4(17), b5(2), b5(4), b5(6), b5(8), b5(9), b5(10), b5(12), b5(13), b5(14), b5(15), b5(19), b3(7). the results showed that there were five rhizobacterial isolates that had the ability to spur plant growth, namely b5(4), b5(8), b4(12), b4(13), and b3(16) isolates of rhizobacteria. the five rhizobacterial isolates had an influence in increasing plant height, leaf number, leaf length, stem diameter, amount of chlorophyll, wet weight, dry weight, and being able to increase soil ph. rhizobacterial isolates b1(9), b1(13), b1(17), b1(20), b3(10), b3(12), b3(16), b4(12), b4(13), b4(5), b4(6), b4(8), b5(10), b5(14), b5(4) and b5(8) are capable of conducting fluorescent activities. isolates b1(7), b1(19), b3(10), b3(12), b3(16), b3(19), b3(9), b4(12), b4(15), b4(5) , b5(13), b5(15), b5(19), b5(8), b5(9) capable of tethering nitrogen. b1(17), b1(7), b2(6), b3(10), b3(12), b3(19), b4(12), b4(15), b4(3), b5(10), b5(13), dan b5(4) were able to resist chloramphenicol antibiotics at a dose of 75 Μg ml-, and isolates b1(13), b1(20), b2(6), b3(12), b3(19), b3(2), b3(9), b4(11), b4(13), b4(15), b4(3), b5(14), b5(15), dan b5(2). resistant to amoxilin and rifampicin antibiotics at doses of 50 Μg ml-1.